The ability to write DNA efficiently is a key necessity for almost all applications in the life science industry. DNA synthesis enables the engineering of biological systems (enzymes, antibodies, bacteria, yeast, plants, cells, etc.) to create new applications, most notably in biotherapeutics, the production of chemicals and materials, agriculture or nanotechnology.
DNA Script is developing a novel enzymatic technology to synthesize DNA that will increase performance in strand length, purity, turnaround time and cost. This blog series reports key technological milestones — initially revealed at the GP-write Conference
in May 2018 — which demonstrate the potential of enzymatic synthesis, even at this early phase in the development of our technology. What to do with enzymatically synthesized primers? First example: PCR
Attaining synthesis of 50-nucleotide strands of DNA is a significant milestone, but we believe using our enzymatically synthesized primers in real conditions is even more important. The result below shows the first successful PCR performed using two enzymatically synthesized primers.
● T7 forward:
● T7 reverse:
Those primers were used to amplify a 373bp insert in a plasmid.